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DNA-RNA

Nuleosyn offers an extensive line of standard phosphoramidites, RNA phosphoramidites, specialty amidites and modifiers, solid supports CPG, reagents and solvents for a wide variety of instruments

Fluo-dT

CAS #
289712-99-8
Packing size
50µmol, 100µmol, 250mg
FluoresceinDeoxythymidineDeoxyribosePhosphoramiditeDNA & RNA SupportsDNA & RNA AnalogsDNA & RNA Nucleosides
Fluo-dT Image 1

Fluorescein is used extensively as a diagnostic tool, commonly used for labelling oligos and peptides. 5'-Fluorescein phosphoramidite contains no 4,4'-dimethoxytrityl (DMT) group and can be added only once at the 5'-terminus, thereby terminating synthesis. Fluorescein-dT can be inserted into the desired sequence as a replacement for a dT residue.

Aspect
White to pale yellow solid
Purity
>95% (LC)
Solubility
Dichloromethane, EtOAc, MeCN
Molecular weight
1425,58
Molecular formula

C79H89N6O17P

IUPAC
6-((6-((E)-3-(1-((2R,4S,5R)-5-((bis(4-methoxyphenyl)(phenyl)methoxy)methyl)-4-(((2-cyanoethoxy)(diisopropylamino)phosphaneyl)oxy)tetrahydrofuran-2-yl)-2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)acrylamido)hexyl)carbamoyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyl bis(2,2-dimethylpropanoate)

A. Kausar, C. J. Mitran, Y. Li, J. M. Gibbs-Davis, Angew. Chem. Int. Ed., 2013, 52, 10577-10581. read article

A. H. El-Sagheer, T. Brown, Int. J. Pept. Res. Ther., 2008, 14, 367-372. read article

 

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