Nuleosyn offers an extensive line of standard phosphoramidites, RNA phosphoramidites, specialty amidites and modifiers, solid supports CPG, reagents and solvents for a wide variety of instruments
N-Quencher-2-CPG1000 (glycolate linker)
Commonly used quenchers encounter important limitations, such as a background increase with TAMRA due to its native fluorescence, or a poor overlap with reporter dyes emitting above 480 nm in the case of DABCYL. N-Quencher-1 and -2 generate heat rather than light, resulting in a lower background fluorescence. In addition, N-Quencher-1 and -2 proved to be excellent quenchers for molecular beacons and dual-labeled probes, thanks to a significant increase in both static and FRET quenching efficiencies. The maximum absorption of N-Quencher-1 (534 nm) and N-Quencher-2 (579 nm) makes them directly superimposable with the maximum emission wavelengths of various fluorescent dyes, enabling a wider choice of reporters, including 6-FAM, TET, HEX, or ROX for multiplexing.
Provided as supported reagents, with a glycolate linker, N-Quencher-1 and N-Quencher-2 can be incorporated at the 3’-terminus of oligonucleotides after cleavage of their DMT group.
Related compound: N-Quencher-1-CPG1000 (glycolate linker)
